Defatted rice bran is mixed with diluted acid solution, the mixture is agitated some hrs. at constant temparature.
After the mixture is filtered, thus filtrate is obtained.
This filtrate is phytin extract solution. (Test-1)
The alkali is added to this filtrate and filtered out, then the precipitation of phytin is obtained. (Test-2)
At the test-1, the effect of kind of acid, conc. of acid, amount of extract sol´n., time of extraction, temp. of extraction, to the extract amount of phytin is tested.
Consequently, the following facts are known.
1. Amount of phytin extract is greater HCI extraction than H₂SO₄ extraction.
2. At 0.3% HCI, the amount of phytin extract is greatest of all HCl extraction.
3. The sufficient amount of acid solution is 8-10 times of amount of defatted rice bran.
4. The time of extraction at room temperature is sufficient 8-12 hrs.
5. When extract temperature is 20-30℃, the amount of phytin extraction is greater of all temp..
6, When defatted rice bran 20 g is shanken with 160 ㎖ of 0.3% HCl for 10 hr. at room temp., in this case the amount of phytin extract is 11.34% of defatted rice bran, it is 93% of theoretical yield.
At the test-2 the effect of kind of precipitation agent, degree of nutralization to the amount of phytin prc,ipitation is tested.
1. Degree of nut. is best at pH 6.8-7.0.
2. When use of Ca(OH)₂ the amount of phytin precipitation is more than use of KOH, NaOH, or NH₄OH.
3. At pH 6.0-7.2, the solubility of phytin is followed.
K-phytate〉 NH₄-phytate〉 Na-phytate〉 Ca-phytate
4. When phytin extract solution is nutralized with Ca(OH)₂ to pH 7.0, the amount of phytin precipitation is 94.78% of theoretical yield.
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